常见的通用引物序列
2011/07/12, 浏览

奥科公司测序部通用引物合成

引物名称

引物序列

 

T7

TAATACGACTCACTATAGGG

 

SP6

5'ATTTAGGTGACACTATAG3'

 

M13F

5'TGTAAAACGACGGCCAGT3'

 

M13R

5'CAGGAAACAGCTATGACC3'

 

T717BASE

5'ACATCCACTTTGCCTTTCTC3'

 

T7TER

5'TGCTAGTTATTGCTCAGCGG3'

 

S.TAG

5'CGAACGCCAGCACATGGACA3'

 

T3

5'ATTAACCCTCACTAAAGGGA3'

 

M13F47

5'CGCCAGGGTTTTCCCAGTCACGAC3'

 

M13R48

5'AGCGGATAACAATTTCACACAGGA3'

 

PGEX5’

5'GGGCTGGCAAGCCACGTTTGGTG3'

 

PGEX3’

5'CCGGGAGCTGCATGTGTCAGAGG3'

 

PEGFPC5’

5'CATGGTCCTGCTGGAGTTCGTG3'

 

PEGFPC3’

5'TATGGCTGATTATGATCAGT3'

 

PEGFPN5’

 5'TGGGAGGTCTATATAAGCAGAG3'

 

PEGFPN3’

 5'CGTCGCCGTCCAGCTCGACCAG3'

 

PBV220F

 5'AAGAAGGGCAGCATTCAAAG3'

 

PBV220R

 5'CTG CGT TCT GAT TTA ATC TG3'

 

5’AOX

5'GACTGGTTCCAATTGACAAGC3'

 

3’AOX

5'GGCAAATGGCATTCTGACAT3'

 

AFACTOR

 5'TAC TAT TGC CAG CAT TGC TGC3'

 

M13(96)

5'CCCTCATAGTTAGCGTAACG3'

 

PCMVF-BD

 5'TCTAAAAGCTGCGGAATTGT3'

 

PCMVR

5'TCCAAACTCATCAATGTATC3'

 

P17110

5TACCACTACAATGGATG3

 

P12584

5TTTTCAGTATCTACGAT3

 

PAS21F

5' TCATCGGAAGCGAGTAG3'

 

PAS21R

5' CGTTTTAAAACCTAAGAGTCAC3'

 

3'AD

5' AGA TGG TGC ACG ATG CAC AG3'

 

3'BD

5'TAA GAG TCA CTT TAA AAT TTG TAT C3'

 

PQE30F

5' TGA GCG GAT AAC AAT TTC AC3'

 

PQE30R

 5' GTT CTG AGG TCA TTA CTG G3'

 

S1

5CAA CGT GAA AAA ATT ATT ATT CGC3

 

S6

5GTA AAT GAA TTT TCT GTA TGA GG3

 

GLP1

5'  TGT ATC TTA TGG TAC TGT AAC TG3'

 

GLP2

5' CTT TAT GTT TTT GGC GTC TTC CA3'

 

RVP3

5' CTA GCA AAA TAG GCT GTC CC3'

 

RVP4

5'GAC GAT AGT CAT GCC CCG CG3'

 

PTRC99CF

5TTG CGC CGA CAT CAT AAC3

 

PTRC99CR

5CTG CGT TCT GAT TTA ATC TG3

 

PIRES2EGFPF

5'GTA GGC GTG TAC GGT GGG AG3'

 

PIRES3EGFPR

5'AAC GCA CAC CGG CCT TAT TC3'

 

PCDNA3.0F2

5'CTC TGG CTA ACT AGA GAA CC3'

 

PCDNA3.0R2

 5'GGC AAC TAG AAG GCA CAG TC3'

 

BAC1

 5'AAC CAT CTC GCA AAT AAA TA 3'

 

BAC2

 5'ACG CAC AGA ATC TAG CGC TT 3'

 

PB42ADF

5'CCAGCCTCTTGCTGAGTGGAGATG3'

 

PB42ADR

5'AAGCCGACAACCTTGATTGGAG3'

 

TRXF

5'CATATGAGCGATAAAATTATTCAC3'

 

TRXR

5'GGATCCCTTGTCATCGTCATCACCA3'

 

pTARGETSEQ

5’ TTA CGC CAA GTT ATT TAG GTG ACA3’

 

pMALC2XF

5TGC GTA CTG CGG TGA TCA AC3

 

pMALC2XR

5CTG CAA GGC GAT TAA GTT GG3

 

pbdgal4f

5'GCCTCTAACATTGAGACAGC3'

 

pbdgal4r

5'AAGAGTTACTCAAGAACAAGAA3'

 

pbi121(35s)

5'GACGCACAATCCCACTATCC3'

 

PEFF

5' TCA AGC CTC AGA CAG TGG TTC3'

 

PCEPF

5' AGA GCT CGT TTA GTG AAC CG3'

 

EBVR

5' GTG GTT TGT CCA AAC TCA TC3'

 

CMVF

5'CGCAAATGGGCGGTAGGCGTG3'

 

CMVR

5'GTTCACGGTGCCCTCC3'

 

PTRCHISAF

5CAT GGT ATG GCT AGC ATG AC3

 

PTRCHISAR

5CAG GCT GAA AAT CTT CTC TC3

 

pDC316 F

 5' -TAA CTT GTT TAT TGC AGC -3'

 

pDC316 A

5' -TAT GCT TAC CGT AAC TTG -3'

 

BGH

5'TAG AAG GCA CAG TCG AGG3'

 

pDC316F

5' acg tgg gta taa gag gcg 3'

 

pDC316R

5' cga tgc tag acg atc cag 3'

 

pshuttle-cmv F

 5'-ggT CTA TAT AAg CAg AgC Tg-3'

 

pshuttle-cmv R

 5'-gTg gTA Tgg CTg ATT ATg ATC Ag-3'

 

GAL4BD

 5'TCA TCG GAA GAG AGT AG 3'

 

TADH

 5'GTA AAT TTC TGG CAA GGT AGA C 3'

 

pDsRed-n5'

GACTTTCCAAAATGTCGTAA

 

T7-EEV

 5'AAG GCT AGA GTA CTT AAT ACG A 3'

 

pfastBac-5'

GTC CGA AAC CAT GTC GTA C

 

pfastBac-3'

CTG ATT ATG ATC CTC TAG TAC TTC

 

PBAD5'

TAT CGC AAC TCT CTA CTG TT

 

PBAD3'

5' AGC CAA GCT GGA GAC CGT TT 3'

 

5'AD

AAT ACC ACT ACA ATG GAT GAT G

 

N-CMV-30

ATA ACC CCG CCC CGT TG

 

C-CMV-24

TTA GGA CAA GGC TGG TGG

 

pBABE_5'

ctt tat cca gcc ctc ac

 

pBABE_3' 

accctaactgacacacattcc

 

P5'

TGC GTA CTG CGG TGA TCA AC

 

P3'

CTG CAA GGC GAT TAA GTT GG

 

 DuetDOWN1

 5'-GATTATGCGGCCGTGTACAA-3'

 

PET Upstream

ATG CGT CCG GCG TAG AGG AT

 

DuetUP2

5'-TTGTACACGGCCGCATAATC-3'

 

pSR 5' Primer

ggaagccttggcttttg

 

SV40 PolyA

5'  TAAGATACATTGATGAGTTTGGAC  3'

 

pCMV5R   

ATTATAgAggACACCTAgTC

 

pCMV5F   

TTCCAAAATgTCgTAATAAC